The Tissue Culture Laboratory
The University of Kansas Higuchi Biosciences Center


    Microscope Images - Examples


    All images on this page were taken using the TCHL’s Nikon TE200 microscope and 3CCD Digital Video Camera.

    Example #1

    Live/Dead Study of Neurons in cell culture, a model of Alzheimer’s disease.

    Phase contrast image of embryonic Live (green) and dead (red) neurons
    rat brain cortical neurons after a toxic stimulus



    Example #2
    Chinese Hamster Ovary (CHO) Cells. The nuclei of the cells have been stained with Hoechst 33258

    Brightfield Images

    10X magnification 20X magnification
    40X magnification 20X magnification plus zoom
    adjustment on the digital camera


    Fluorescence Images

    20X magnification 40X magnification


    Example #3 Transfection of a gene for Green Fluorescent Protein into cos7 cells. The cos7 cells were transfected using the TCHL’s BTX ECM 2001 Electroporator/electrofusion unit.

    Brightfield image of the cos7 cells
    Fluorescence image of the same cells showing expression of Green Fluorescent Protein.

     

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    Last Update 8/22/06