John
Stobaugh
Research Interests
Bioanalytical chemistry, organic analytical chemistry, liquid chromatography, capillary electrophoresis, capillary electrochromatography, fluorescence, laser-induced fluorescence and electrochemical detection, analysis of drugs, analysis of endogenous substances, peptides and anticancer agents, development of liquid and solid-phase derivatization reagents, chiral separations and surface modified capillariesProfessor Stobaugh and his research group are currently emphasizing the development of drugs and their metabolites, peptides and anticancer agents in biological samples. In the reagent area, novel solution and solid-phase chemistries are being developed that can be used in conjunction with state-of-the-art separation techniques, such as microcolumn liquid chromatography, capillary electrophoresis and capillary electrochromatography. Specifically, pre-separation reagents are being developed for the fluorogenic and electrogenic derivatization of amines, amino acids, peptides and drug substances. The resulting products are separated by one of the aforementioned techniques prior to detection by high sensitivity techniques such as fluorescence, laser-induced fluorescence or electrochemistry.
Research in capillary electrophoresis separations includes the separation of
drug stereoisomers via the use of chemically modified cyclodextrin selectors
and the surface modification of capillary surfaces for the prevention
of protein adsorption and the control of electroosmotic flow. More recently,
emphasis has been placed on the evaluation of capillary electrochromatography
as a technique for pharmaceutical and biomedical analysis. Current efforts
include the development of rugged packing techniques and the evaluation
and development of chromatographic phases with unique applicability to
this newly emerging separation technique.
Representative publications
M. J. Rose, S. M. Lunte, R. G. Carlson, and J. F. Stobaugh. Amino acid and peptide analysis using derivatization with p-nitrophenol-2,5-dihydroxyphenylacetate bis-tetrahydropyranyl ether and capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal 30: 1851-9 (2003).
J. Z. Yang, K. C. Bastian, R. D. Moore, J. F. Stobaugh, and R. T. Borchardt.
Quantitative analysis of a model opioid peptide and its cyclic prodrugs
in rat plasma using high-performance liquid chromatography with fluorescence
and tandem mass spectrometric detection. J Chromatogr B Analyt Technol
Biomed Life Sci 780: 269-81 (2002).
C. Ampasavate, G. A. Chandorkar, D. G. Vande Velde, J. F. Stobaugh, and
K. L. Audus. Transport and metabolism of opioid peptides across BeWo cells,
an in vitro model of the placental barrier. Int J Pharm 233: 85-98 (2002).
S. E. Moring, O. S. Wong, and J. F. Stobaugh. Target specific sample preparation
from aqueous extracts with molecular imprinted polymers. J Pharm Biomed
Anal 27: 719-28 (2002).
M. J. Rose, S. M. Lunte, R. G. Carlson, and J. F. Stobaugh. Transformation
of analytes for electrochemical detection: a review of chemical and physical
approaches. Adv Chromatogr 41: 203-48 (2001).
P. D. Angus, C. W. Demarest, T. Catalano, and J. F. Stobaugh. Aspects
of column fabrication for packed capillary electrochromatography. J Chromatogr
A 887: 347-65 (2000).