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Russ Middaugh

Office: B051 Smissman Laboratories Phone: (785)864-5813
FAX: (785)864-5736

(email: middaugh@ku.edu)

Research Interests

The majority of our research focuses in three areas. The first involves the development of vehicles to deliver polynucleotides for gene therapy. To this end, we are: (a) exploring the use of physical methods such as infrared spectroscopy, circular dichroism, fluorescence, light-scattering, calorimetry and related techniques to characterize viral and nonviral gene delivery complexes, (b) studying the cellular barriers to vehicle transport and (c) preparing novel gene delivery systems.

The second area involves studies of proteins that transport across cellular barriers by currently unknown mechanisms. These efforts involve investigations of the structure, stability and membrane activity of a wide variety of proteins of pharmaceutical interest including growth factors and Factor VIII.

The other major area of interest is vaccines. We are currently exploring structural aspects of the delivery of both proteins and polynucleotides as vaccines and the use of adjuvants to enhance immunogenicity in these systems.

Representative Publications

S. Choosakoonkriang, C. M. Wiethoff, G. S. Koe, J. G. Koe, T. J. Anchordoquy, and C. R. Middaugh. An infrared spectroscopic study of the effect of hydration on cationic lipid/DNA complexes. J Pharm Sci 92: 115-30 (2003).

L. A. Kueltzo, J. Osiecki, J. Barker, W. L. Picking, B. Ersoy, W. D. Picking, and C. R. Middaugh. Structure-Function Analysis of Invasion Plasmid Antigen C (IpaC) from Shigella flexneri. J Biol Chem 278: 2792-8 (2003).

C. M. Wiethoff, M. L. Gill, G. S. Koe, J. G. Koe, and C. R. Middaugh. The structural organization of cationic lipid-DNA complexes. J Biol Chem 277: 44980-7 (2002).

D. S. Maclean, Q. Qian, and C. R. Middaugh. Stabilization of proteins by low molecular weight multi-ions. J Pharm Sci 91: 2220-9 (2002).

D. B. Volkin, G. Sanyal, C. J. Burke, and C. R. Middaugh. Preformulation studies as an essential guide to formulation development and manufacture of protein pharmaceuticals. Pharm Biotechnol 14: 1-46 (2002).

C. Y. Huang, S. S. Ma, S. Lee, R. Radhakrishnan, C. S. Braun, S. Choosakoonkriang, C. M. Wiethoff, B. A. Lobo, and C. R. Middaugh. Enhancements in gene expression by the choice of plasmid DNA formulations containing neutral polymeric excipients. J Pharm Sci 91: 1371-81 (2002).

P. O. Souillac, C. R. Middaugh, and J. H. Rytting. Investigation of protein/carbohydrate interactions in the dried state. 2. Diffuse reflectance FTIR studies. Int J Pharm 235: 207-18 (2002).

B. A. Lobo, S. A. Rogers, S. Choosakoonkriang, J. G. Smith, G. Koe, and C. R. Middaugh. Differential scanning calorimetric studies of the thermal stability of plasmid DNA complexed with cationic lipids and polymers. J Pharm Sci 91: 454-66 (2002).

S. A. Tatulian, B. Chen, J. Li, S. Negash, C. R. Middaugh, D. J. Bigelow, and T. C. Squier. The inhibitory action of phospholamban involves stabilization of alpha-helices within the Ca-ATPase. Biochemistry 41: 741-51 (2002).

P. O. Souillac, H. R. Costantino, C. R. Middaugh, and J. H. Rytting. Investigation of protein/carbohydrate interactions in the dried state. 1. Calorimetric studies. J Pharm Sci 91: 206-16 (2002).

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